Human DBP (Vitamin D-Binding Protein) Antibody Pair (STJAP00230)
General Information
| Short Description | This DBP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | GC |
| Gene ID | 2638 |
| Uniprot ID | VTDB_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human DBP protein.Detection Immunogen: Recombinant Human DBP protein. |
Additional Info
| Post Translational Modifications | Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated. |
| Function | Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. |
| Protein Name | Vitamin D-Binding ProteinDbpVdbGc Protein-Derived Macrophage Activating FactorGc-MafGcmafGc-GlobulinGroup-Specific ComponentGcVitamin D-Binding Protein-Macrophage Activating FactorDbp-Maf |
| Database Links | Reactome: R-HSA-196791 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Vitamin D-Binding Protein antibody pairDbp antibody pairVdb antibody pairGc Protein-Derived Macrophage Activating Factor antibody pairGc-Maf antibody pairGcmaf antibody pairGc-Globulin antibody pairGroup-Specific Component antibody pairGc antibody pairVitamin D-Binding Protein-Macrophage Activating Factor antibody pairDbp-Maf antibody pairGC antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human DBP (Vitamin D-Binding Protein) Antibody Pair (STJAP00230)
This antibody pair detects human DBP (Vitamin D-Binding Protein), corresponding to UniProt entry P02774. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated at 0.5 mg/mL, while the detection antibody is biotin-conjugated at 0.5 mg/mL. Both are rabbit polyclonal antibodies purified by Protein A or G, and each is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant human DBP protein. No further detail on the specific immunogen region is provided in the current product documentation.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.