Human DARC (Duffy Blood Group Chemokine Receptor) Sandwich ELISA Kit Ready-To-Use Kit (STJE0014735)
SPECIFICATIONS
General Information
| Short Description | This DARC Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.062ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | ACKR1 |
| Gene ID | 2532 |
| Uniprot ID | ACKR1_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. |
| Post Translational Modifications | Sulfation at Tyr-41 facilitates interaction with MGSA/CXCL1, RANTES/CCL5 and MCP-1/CCL2 but not IL8/CXCL8. Sulfation at Tyr-30 facilitates interaction with IL8/CXCL8. (Microbial infection) Sulfation at Tyr-41 facilitates interaction with Plasmodium vivax Duffy receptor (PVDR). Sulfation at Tyr-30/Tyr-41 and Tyr-41 alone increases binding affinity of Plasmodium vivax parasites and likely promotes invasion of red blood cells. (Microbial infection) Sulfation at Tyr-41 facilitates interaction with Plasmodium knowlesi Duffy receptor alpha form (DBPalpha). Sulfation at Tyr-30/Tyr-41 and Tyr-41 alone increases binding affinity of Plasmodium knowlesi parasites and likely promotes invasion of red blood cells. |
| Function | Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes.when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels. (Microbial infection) Acts as a receptor for the malaria parasite Plasmodium vivax. (Microbial infection) Acts as a receptor for the malaria parasite Plasmodium knowlesi. |
| Protein Name | Atypical Chemokine Receptor 1Duffy Antigen Receptor For ChemokinesDuffy Antigen/Chemokine ReceptorDuffy Blood Group AntigenFy GlycoproteinGpfyGlycoprotein DPlasmodium Vivax ReceptorCd Antigen Cd234 |
| Database Links | Reactome: R-HSA-375276 |
| Cellular Localisation | Early EndosomeRecycling EndosomeMembraneMulti-Pass Membrane ProteinPredominantly Localizes To Endocytic VesiclesAnd Upon Stimulation By The Ligand Is Internalized Via CaveolaeOnce InternalizedThe Ligand Dissociates From The ReceptorAnd Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane |
| Alternative ELISA Names | Atypical Chemokine Receptor 1 ELISA kitDuffy Antigen Receptor For Chemokines ELISA kitDuffy Antigen/Chemokine Receptor ELISA kitDuffy Blood Group Antigen ELISA kitFy Glycoprotein ELISA kitGpfy ELISA kitGlycoprotein D ELISA kitPlasmodium Vivax Receptor ELISA kitCd Antigen Cd234 ELISA kitACKR1 ELISA kitDARC ELISA kitFY ELISA kitGPD ELISA kit |
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Information sourced from Uniprot.org