Human DAO (D-Amino-Acid Oxidase) Antibody Pair (STJAP00468)
General Information
| Short Description | This DAO antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | DAO |
| Gene ID | 1610 |
| Uniprot ID | OXDA_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human DAO protien.Detection Immunogen: Recombinant Human DAO protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylated in the cerebellum.probably not by PRKACA, PRKCA or PRKCE. May be S-nitrosylated, which partially inactivates the enzyme. |
| Function | Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Required to catabolize D-amino acids synthesized endogenously, of gastrointestinal bacterial origin or obtained from the diet, and to use these as nutrients. Regulates the level of D-amino acid neurotransmitters in the brain, such as D-serine, a co-agonist of N-methyl D-aspartate (NMDA) receptors, and may modulate synaptic transmission. Catalyzes the first step of the racemization of D-DOPA to L-DOPA, for possible use in an alternative dopamine biosynthesis pathway. Also catalyzes the first step of the chiral inversion of N(gamma)-nitro-D-arginine methyl ester (D-NNA) to its L-enantiomer L-NNA that acts as a nitric oxide synthase inhibitor. The hydrogen peroxide produced in the reaction provides protection against microbial infection.it contributes to the oxidative killing activity of phagocytic leukocytes and protects against bacterial colonization of the small intestine. Enzyme secreted into the lumen of the intestine may not be catalytically active and could instead be proteolytically cleaved into peptides with antimicrobial activity. The hydrogen peroxide produced in the reaction may also play a role in promoting cellular senescence in response to DNA damage. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. |
| Protein Name | D-Amino-Acid OxidaseDaaoDamoxDao |
| Database Links | Reactome: R-HSA-389661Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome MatrixCytoplasmCytosolPresynaptic Active ZoneSecretedTransiently Present In The Cytosol Before Being Delivered To The PeroxisomesIn The CerebellumA Fraction Of Protein Localizes To The Presynaptic Active ZoneWhere Its Activity Is Regulated By Protein BsnSecreted Into The Lumen Of The Small Intestine |
| Alternative Antibody Pair Names | D-Amino-Acid Oxidase antibody pairDaao antibody pairDamox antibody pairDao antibody pairDAO antibody pairDAMOX antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human DAO (D-Amino-Acid Oxidase) Antibody Pair (STJAP00468)
This antibody pair detects human D-Amino-Acid Oxidase (DAO, UniProt P14920) and has confirmed reactivity with human samples only. Both the capture and detection antibodies were raised against recombinant human DAO protein.
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), while the detection antibody is biotinylated and supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5). Both antibodies are rabbit-derived and purified by antigen affinity.
This antibody pair has been validated for use in ELISA. It is intended for the detection of human DAO in serum, plasma, and other biological fluids.
Validated sample types include human serum, plasma, and other biological fluids. This product is for research use only and is not validated for diagnostic or therapeutic applications.
Both the capture and detection antibodies should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.