Human Cytokeratin 18 (Cytokeratin 18) Antibody Pair (STJAP00861)
General Information
| Short Description | This Cytokeratin 18 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R01-2N-4/R04-9E-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | KRT18 |
| Gene ID | 3875 |
| Uniprot ID | K1C18_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. |
| Post Translational Modifications | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
| Function | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Protein Name | Keratin - Type I Cytoskeletal 18Cell Proliferation-Inducing Gene 46 ProteinCytokeratin-18Ck-18Keratin-18K18 |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | Nucleus MatrixCytoplasmPerinuclear RegionNucleusNucleolus |
| Alternative Antibody Pair Names | Keratin - Type I Cytoskeletal 18 antibody pairCell Proliferation-Inducing Gene 46 Protein antibody pairCytokeratin-18 antibody pairCk-18 antibody pairKeratin-18 antibody pairK18 antibody pairKRT18 antibody pairCYK18 antibody pairPIG46 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Cytokeratin 18 (Cytokeratin 18) Antibody Pair (STJAP00861)
This antibody pair detects human Cytokeratin 18, corresponding to UniProt entry P05783. Reactivity has been confirmed for human samples only.
This antibody pair is validated for use in ELISA. It is intended for use in sandwich ELISA configurations using the two provided monoclonal clones as capture and detection antibodies.
Both antibodies are rabbit-derived monoclonals. The clone IDs are R01-2N-4 and R04-9E-5, and both are IgG isotype.
Both antibodies are supplied unconjugated, formulated in 0.01M PBS at pH 7.2, and purified by affinity purification. Concentration and recommended working dilutions are to be determined by the end user.
Both antibodies were raised against a recombinant protein immunogen corresponding to human Cytokeratin 18. No specific immunogen region is indicated in the product details.
The antibodies should be stored at 4°C for short-term use. For long-term storage, they should be aliquoted and kept at -20°C. Repeated freeze/thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.