Human Cystatin B protein (Recombinant) (His-Tag) (STJP019257)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP019257
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General Information

Short DescriptionRecombinant-Human Cystatin B-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsEnzyme Activity/SDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration1 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin< 1 EU per 1ug of protein (determined by LAL method)
ImmunoreactivityThe IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Target Information

Gene SymbolCSTB
Gene ID1476
Uniprot IDCYTB_HUMAN
Accession NumberNP_000091
ImmunogenHuman
Immunogen Region1-98aa
Immunogen SequenceMGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF

Additional Info

Function This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Protein Name Cystatin-B
Cpi-B
Liver Thiol Proteinase Inhibitor
Stefin-B
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Nucleus
Alternative Protein Names Cystatin-B protein
Cpi-B protein
Liver Thiol Proteinase Inhibitor protein
Stefin-B protein
CSTB protein
CST6 protein
STFB protein

Information sourced from Uniprot.org

Citations

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