Human Cystatin A protein (Recombinant) (STJP000307)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Cystatin A-CSTA (ILE 2-PHE98) mature chain including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Cystatin A-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to be 10nM NA |
Target Information
| Gene Symbol | CSTA |
| Gene ID | 1475 |
| Uniprot ID | CYTA_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Cystatin A-CSTA (ILE 2-PHE98) mature chain including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ILE 2-PHE98 |
Additional Info
| Tissue Specificity | Expressed in the skin throughout the epidermis. |
| Function | This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. |
| Protein Name | Cystatin-ACystatin-AsStefin-A Cleaved Into - Cystatin-A - N-Terminally Processed |
| Database Links | Reactome: R-HSA-6809371 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Cystatin-A proteinCystatin-As proteinStefin-A Cleaved Into - Cystatin-A - N-Terminally Processed proteinCSTA proteinSTF1 proteinSTFA protein |
Information sourced from Uniprot.org