Human CYP11B1 (Cytochrome P450 11B1) Sandwich ELISA Kit (STJE0008302)
SPECIFICATIONS
General Information
| Short Description | This CYP11B1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.074ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | CYP11B1 |
| Gene ID | 1584 |
| Uniprot ID | C11B1_HUMAN |
| Sample Type | serum, plasma, tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in the zona fasciculata/reticularis of the adrenal cortex. |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b1 - MitochondrialCyp11b1Cypxib1Cytochrome P-450c11Cytochrome P450c11Steroid 11-Beta-Hydroxylase - Cyp11b1 |
| Database Links | Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579017 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative ELISA Names | Cytochrome P450 11b1 - Mitochondrial ELISA kitCyp11b1 ELISA kitCypxib1 ELISA kitCytochrome P-450c11 ELISA kitCytochrome P450c11 ELISA kitSteroid 11-Beta-Hydroxylase - Cyp11b1 ELISA kitCYP11B1 ELISA kitS11BH ELISA kit |
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Information sourced from Uniprot.org