Human/Cynomolgus/Rhesus macaque CD28 protein (Recombinant) (N-hFc) (STJP020003)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn19-Pro152) of Human/Cynomolgus/Rhesus-macaque CD28 (Accession #P10747-1) fused with hFc tag at t
General Information
| Short Description | Recombinant-Human/Cynomolgus/Rhesus macaque CD28-N-hFc protein was developed in hek293 cells using the region Asn19-Pro152. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD28 |
| Gene ID | 940 |
| Uniprot ID | CD28_HUMAN |
| Immunogen | Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn19-Pro152) of Human/Cynomolgus/Rhesus-macaque CD28 (Accession #P10747-1) fused with hFc tag at t |
| Immunogen Region | Asn19-Pro152 |
| Immunogen Sequence | NKILVKQSPMLVAYDNAVNL SCKYSYNLFSREFRASLHKG LDSAVEVCVVYGNYSQQLQV YSKTGFNCDGKLGNESVTFY LQNLYVNQTDIYFCKIEVMY PPPYLDNEKSNGTIIHVKGK HLCPSPLFPGPSKP |
Additional Info
| Tissue Specificity | Expressed in T-cells and plasma cells, but not in less mature B-cells. |
| Post Translational Modifications | Phosphorylated by LCK. Dephosphorylated by PTPN11. Isoform 3: Tyrosine phosphorylated induced by CD40LG. |
| Function | Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. Isoform 3: Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. |
| Protein Name | T-Cell-Specific Surface Glycoprotein Cd28Tp44Cd Antigen Cd28 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-164939Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell Surface |
| Alternative Protein Names | T-Cell-Specific Surface Glycoprotein Cd28 proteinTp44 proteinCd Antigen Cd28 proteinCD28 protein |
Information sourced from Uniprot.org