Human CXCR3 (C-X-C chemokine receptor type 3) CLIA Kit for Quantitative Detection

Name: Human CXCR3 (C-X-C chemokine receptor type 3) CLIA Kit for Quantitative Detection
SKU: STJC0000428
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000428

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General Information

Short Description	This human CXCR3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Target Information

Gene Symbol	CXCR3
Gene ID	2833
Uniprot ID	CXCR3_HUMAN
Specificity	This kit recognizes Human CXCR3 in samples. No significant cross-reactivity or interference between Human CXCR3 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Tissue Specificity	Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).
Post Translational Modifications	Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction. N-glycosylated.
Function	Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Binds to CCL21. Probably promotes cell chemotaxis response. Upon activation by PF4, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Isoform 2: Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway. Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis. Isoform 3: Mediates the activity of CXCL11.
Protein Name	C-X-C Chemokine Receptor Type 3 Cxc-R3 Cxcr-3 Ckr-L2 G Protein-Coupled Receptor 9 Interferon-Inducible Protein 10 Receptor Ip-10 Receptor Cd Antigen Cd183
Database Links	Reactome: R-HSA-380108 Reactome: R-HSA-418594
Cellular Localisation	Isoform 1: Cell Membrane Multi-Pass Membrane Protein Isoform 2: Cell Membrane
Alternative CLIA Names	C-X-C Chemokine Receptor Type 3 CLIA kit Cxc-R3 CLIA kit Cxcr-3 CLIA kit Ckr-L2 CLIA kit G Protein-Coupled Receptor 9 CLIA kit Interferon-Inducible Protein 10 Receptor CLIA kit Ip-10 Receptor CLIA kit Cd Antigen Cd183 CLIA kit CXCR3 CLIA kit GPR9 CLIA kit
Specificity	This kit recognizes Human CXCR3 in samples. No significant cross-reactivity or interference between Human CXCR3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-107	100
EDTA plasma(n=8)	86-99	93
Cell culture media(n=8)	100-114	106

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	110.69	194.55	842.02	110.24	180.00	856.08
Standard deviation	11.18	18.40	56.67	9.89	15.14	88.09
CV (%)	10.10	9.46	6.73	8.97	8.41	10.29

Citations

View product citations for antibody STJC0000428 on CiteAb