Human CXCL13 (C-X-C motif chemokine 13) Sandwich ELISA (STJE0001954)

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STJE0001954

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This CXCL13 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CXCL13
Gene ID: 10563
Uniprot ID: CXL13_HUMAN
Specificity: This kit recognizes Human BLC in samples. No significant cross-reactivity or interference between Human BLC and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
Protein Name C-X-C Motif Chemokine 13
Angie
B Cell-Attracting Chemokine 1
Bca-1
B Lymphocyte Chemoattractant
Cxc Chemokine Blc
Small-Inducible Cytokine B13
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative ELISA Names C-X-C Motif Chemokine 13 ELISA kit
Angie ELISA kit
B Cell-Attracting Chemokine 1 ELISA kit
Bca-1 ELISA kit
B Lymphocyte Chemoattractant ELISA kit
Cxc Chemokine Blc ELISA kit
Small-Inducible Cytokine B13 ELISA kit
CXCL13 ELISA kit
BCA1 ELISA kit
BLC ELISA kit
SCYB13 ELISA kit
Specificity This kit recognizes Human BLC in samples. No significant cross-reactivity or interference between Human BLC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 95
EDTA plasma (n=8) 94-106 100
Cell culture media (n=8) 86-98 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.68 153.91 407.63 44.67 160.79 424.18
Standard deviation 3.09 7.23 13.57 2.61 6.45 14.21
CV (%) 6.48 4.70 3.33 5.84 4.01 3.35
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance