Human CTSL (Procathepsin L) Antibody Pair (STJAP00158)
General Information
| Short Description | This CTSL antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | CTSL |
| Gene ID | 1514 |
| Uniprot ID | CATL1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human CTSL protein.Detection Immunogen: Recombinant Human CTSL protein. |
Additional Info
| Function | Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation. Secreted form generates endostatin from COL18A1. Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation. Required for maximal stimulation of steroidogenesis by TIMP1. (Microbial infection) In cells lacking TMPRSS2 expression, facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via a slow acid-activated route with the proteolysis of coronavirus spike (S) glycoproteins in lysosome for entry into host cell. Proteolysis within lysosomes is sufficient to activate membrane fusion by coronaviruses SARS-CoV and EMC (HCoV-EMC) S as well as Zaire ebolavirus glycoproteins. Isoform 2: Functions in the regulation of cell cycle progression through proteolytic processing of the CUX1 transcription factor. Translation initiation at downstream start sites allows the synthesis of isoforms that are devoid of a signal peptide and localize to the nucleus where they cleave the CUX1 transcription factor and modify its DNA binding properties. |
| Protein Name | Procathepsin LCathepsin L1Major Excreted ProteinMep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain |
| Database Links | Reactome: R-HSA-1236977Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-8939242Reactome: R-HSA-9678110Reactome: R-HSA-9694614 |
| Cellular Localisation | LysosomeApical Cell MembranePeripheral Membrane ProteinExtracellular SideCytoplasmic VesicleSecretory VesicleChromaffin GranuleSecretedExtracellular SpaceLocalizes To The Apical Membrane Of Thyroid Epithelial CellsReleased At Extracellular Space By Activated Dendritic Cells And MacrophagesIsoform 2: NucleusTranslation Initiation At Downstream Start Sites Allows The Synthesis Of Isoforms That Are Devoid Of A Signal Peptide And Do Not Transit Through The Endoplasmic Reticulum To Localize To The NucleusNuclear Location Varies During The Cell CycleWith Higher Levels During S Phase |
| Alternative Antibody Pair Names | Procathepsin L antibody pairCathepsin L1 antibody pairMajor Excreted Protein antibody pairMep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain antibody pairCTSL antibody pairCTSL1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CTSL (Procathepsin L) Antibody Pair (STJAP00158)
This antibody pair detects human CTSL, also known as Procathepsin L, corresponding to UniProt entry P07711. Reactivity is confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are polyclonal antibodies raised in goat, supplied at 0.5 mg/mL, and purified by antigen affinity chromatography.
Both the capture and detection antibodies are formulated in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are specified for this product.
Both the capture and detection antibodies were raised against recombinant human CTSL protein. No specific immunogen region is stated in the product details.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.