• Human CTSD (Cathepsin D) CLIA Kit (STJC0000361)

Human CTSD (Cathepsin D) CLIA Kit (STJC0000361)

SKU:
STJC0000361

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human CTSD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CTSD
Gene ID: 1509
Uniprot ID: CATD_HUMAN
Specificity: This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post Translational Modifications N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Function Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Protein Name Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-2022377
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Reactome: R-HSA-77387
Reactome: R-HSA-9018519
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
In Aortic Samples
Detected As An Extracellular Protein Loosely Bound To The Matrix
Alternative CLIA Names Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain CLIA kit
CTSD CLIA kit
CPSD CLIA kit
Specificity This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-117 107
EDTA plasma(n=8) 100-113 107
Cell culture media(n=8) 97-109 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 47.80 135.43 338.17 49.78 136.96 323.89
Standard deviation 4.88 12.92 36.22 6.05 13.37 31.81
CV (%) 10.21 9.54 10.71 12.15 9.76 9.82
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance