Human CTSD (Cathepsin D) CLIA Kit for Quantitative Detection

Name: Human CTSD (Cathepsin D) CLIA Kit for Quantitative Detection
SKU: STJC0000361
Availability: InStock

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SPECIFICATIONS

STJC0000361

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Short Description	This human CTSD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	CTSD
Gene ID	1509
Uniprot ID	CATD_HUMAN
Specificity	This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post Translational Modifications	N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Function	Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Protein Name	Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-2022377 Reactome: R-HSA-2132295 Reactome: R-HSA-6798695 Reactome: R-HSA-77387 Reactome: R-HSA-9018519
Cellular Localisation	Lysosome Melanosome Secreted Extracellular Space Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv In Aortic Samples Detected As An Extracellular Protein Loosely Bound To The Matrix
Alternative CLIA Names	Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain CLIA kit CTSD CLIA kit CPSD CLIA kit
Specificity	This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	47.80	135.43	338.17	49.78	136.96	323.89
Standard deviation	4.88	12.92	36.22	6.05	13.37	31.81
CV (%)	10.21	9.54	10.71	12.15	9.76	9.82