Human CTSD (Cathepsin D Cleaved Into-Cathepsin D Light Chain-Cathepsin D Heavy Chain) Antibody Pair (STJAP00474)
General Information
| Short Description | This CTSD antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | CTSD |
| Gene ID | 1509 |
| Uniprot ID | CATD_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human CTSD protien.Detection Immunogen: Recombinant Human CTSD protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified. |
| Function | Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. |
| Protein Name | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-2022377Reactome: R-HSA-2132295Reactome: R-HSA-6798695Reactome: R-HSA-77387Reactome: R-HSA-9018519 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIn Aortic SamplesDetected As An Extracellular Protein Loosely Bound To The Matrix |
| Alternative Antibody Pair Names | Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain antibody pairCTSD antibody pairCPSD antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CTSD (Cathepsin D Cleaved Into-Cathepsin D Light Chain-Cathepsin D Heavy Chain) Antibody Pair (STJAP00474)
This antibody pair detects human Cathepsin D (CTSD, UniProt P07339), including the cleaved forms comprising the Cathepsin D light chain and heavy chain. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are mouse-derived, purified by Protein A or G, and supplied at 0.5 mg/mL.
This pair is validated for use in ELISA. It is suitable for measuring CTSD in serum, plasma, and other biological fluids.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both the capture and detection antibodies were raised against recombinant human CTSD protein. No further details on the specific immunogen region are provided in the product specifications.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.