Human Cripto-1/TDGF1 protein (Recombinant) (C-His) (STJP019814)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Cripto-1/TDGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu31-Thr172) of human Cripto/TDGF1 (Accession #NP_003203) fused with a 6ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Cripto-1/TDGF1-C-His protein was developed in hek293 cells using the region Leu31-Thr172. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CRIPTO |
| Gene ID | 6997 |
| Uniprot ID | TDGF1_HUMAN |
| Immunogen | Recombinant Human Cripto-1/TDGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu31-Thr172) of human Cripto/TDGF1 (Accession #NP_003203) fused with a 6ΓHis tag at the C-terminus. |
| Immunogen Region | Leu31-Thr172 |
| Immunogen Sequence | LGHQEFARPSRGYLAFRDDS IWPQEEPAIRPRSSQRVPPM GIQHSKELNRTCCLNGGTCM LGSFCACPPSFYGRNCEHDV RKENCGSVPHDTWLPKKCSL CKCWHGQLRCFPQAFLPGCD GLVMDEHLVASRTPELPPSA RT |
Additional Info
| Tissue Specificity | Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung. |
| Post Translational Modifications | The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form. |
| Function | GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. |
| Protein Name | Protein CriptoCripto - Egf-Cfc Family MemberCripto-1 Growth FactorCrgfEpidermal Growth Factor-Like Cripto Protein Cr1Teratocarcinoma-Derived Growth Factor 1 |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1433617Reactome: R-HSA-2892247 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorSecretedReleased From The Cell Membrane By Gpi Cleavage |
| Alternative Protein Names | Protein Cripto proteinCripto - Egf-Cfc Family Member proteinCripto-1 Growth Factor proteinCrgf proteinEpidermal Growth Factor-Like Cripto Protein Cr1 proteinTeratocarcinoma-Derived Growth Factor 1 proteinCRIPTO proteinCRIPTO-1 proteinTDGF1 protein |
Information sourced from Uniprot.org