Human CPB2 (Carboxypeptidase B2) Sandwich ELISA Kit (STJE0003459)
SPECIFICATIONS
General Information
| Short Description | This CPB2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 3ng/mL |
| Detection Limit | 5~320ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | CPB2 |
| Gene ID | 1361 |
| Uniprot ID | CBPB2_HUMAN |
| Specificity | This kit recognizes Human TAFI in samples. No significant cross-reactivity or interference between Human TAFI and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. N-glycan at Asn-108: Hex5HexNAc4. |
| Function | Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. |
| Protein Name | Carboxypeptidase B2Carboxypeptidase UCpuPlasma Carboxypeptidase BPcpbThrombin-Activable Fibrinolysis InhibitorTafi |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-977606 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Carboxypeptidase B2 ELISA kitCarboxypeptidase U ELISA kitCpu ELISA kitPlasma Carboxypeptidase B ELISA kitPcpb ELISA kitThrombin-Activable Fibrinolysis Inhibitor ELISA kitTafi ELISA kitCPB2 ELISA kit |
| Specificity | This kit recognizes Human TAFI in samples. No significant cross-reactivity or interference between Human TAFI and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-97 | 91 |
| EDTA plasma (n=8) | 94-110 | 100 |
| Cell culture media (n=8) | 91-107 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 14.87 | 49.02 | 140.43 | 13.61 | 44.71 | 139.44 |
| Standard deviation | 0.83 | 2.18 | 5.50 | 0.74 | 1.87 | 7.33 |
| CV (%) | 5.58 | 4.45 | 3.92 | 5.44 | 4.18 | 5.26 |