This COPT1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.061ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain. Proteolytic cleavage, leading to a truncated form, is facilitated by SLC31A2 and initiated preferentially by CTSL and to a minor extend by CTSB in endolysosomal compartments. In vitro, is cleaved by CTSL/cathepsin L between residues 8 and 9 from the amino terminus. A post-CTSL/cathepsin L processing occurs to yield to the fully truncated form. Sulfenylated at Cys-189 after stimulation with VEGFA, which induces SLC31A1-KDR disulfide bond formation and their co-internalization to early endosomes, driving to a sustained VEGFR2 signaling.
Function
High affinity copper uptake protein 1: Uniporter that mediates the transport of copper(1+) from the extracellular space to the cytoplasm, across the plasma membrane and delivers directly copper(1+) to specific chaperone such as ATOX1, via a copper(1+)- mediated transient interaction between the C-terminal domain and a copper(1+) chaperone, thus controlling intracellular copper(1+) levels. May function in copper(1+) import from the apical membrane thus may drive intestinal copper absorption. The copper(1+) transport mechanism is sodium-independent, saturable and of high-affinity. Also mediates the uptake of silver(1+). May function in the influx of the platinum-containing chemotherapeutic agents. The platinum-containing chemotherapeutic agents uptake is saturable. In vitro, mediates the transport of cadmium(2+) into cells. Also participates in the first step of copper(2+) acquisition by cells through a direct transfer of copper(2+) from copper(2+) carriers in blood, such as ALB to the N-terminal domain of SLC31A1, leading to copper(2+) reduction and probably followed by copper(1+) stabilization. In addition, functions as a redox sensor to promote angiogenesis in endothelial cells, in a copper(1+) transport independent manner, by transmitting the VEGF-induced ROS signal through a sulfenylation at Cys-189 leadin g to a subsequent disulfide bond formation between SLC31A1 and KDR. The SLC31A1-KDR complex is then co-internalized to early endosomes, driving a sustained VEGFR2 signaling. Truncated CTR1 form: Mobilizes copper(1+) out of the endosomal compartment, making copper(1+) available for export out of the cells.
Protein Name
High Affinity Copper Uptake Protein 1Copper Transporter 1Hctr1Solute Carrier Family 31 Member 1 Cleaved Into - Truncated Ctr1 Form
Cell MembraneMulti-Pass Membrane ProteinEarly Endosome MembraneRecycling Endosome MembraneApical Cell MembraneLate Endosome MembraneBasolateral Cell MembraneThe Localization Is Controlled By The Intra And Extra-Cellular Copper ConcentrationUnder Conditions Of Elevated Extracellular Copper ConcentrationsIt Is Rapidly Internalized By Endocytosis From The Plasma Membrane By A Clathrin- And Dynamin-Mediated Process And Degradated In Order To Prevent Intracellular Copper Accumulation And To Reduce The Transport Of The Copper Across The MembraneThe Internalized Slc31a1 Is Then Localized In Early EndosomesAndUpon A Low Extracellular Copper ConcentrationsIt Is Transported Back To The Plasma Membrane In A Rab11a-Dependent Recycling PathwayLocalizes To The Apical Membrane In Intestinal Epithelial CellsMainly Localized On The Basolateral Side Of Renal Tubular CellsLocalizes To The Neuronal Cell Body Plasma Membranes
Alternative ELISA Names
High Affinity Copper Uptake Protein 1 ELISA kitCopper Transporter 1 ELISA kitHctr1 ELISA kitSolute Carrier Family 31 Member 1 Cleaved Into - Truncated Ctr1 Form ELISA kitSLC31A1 ELISA kitCOPT1 ELISA kitCTR1 ELISA kit
