Human COL1A1 (Collagen alpha-1 (I) chain) Sandwich ELISA Kit (STJE0002542)
SPECIFICATIONS
General Information
| Short Description | This COL1A1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.19ng/mL |
| Detection Limit | 0.31~20ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | COL1A1 |
| Gene ID | 1277 |
| Uniprot ID | CO1A1_HUMAN |
| Specificity | This kit recognizes Human COL1 Alpha 1 in samples. No significant cross-reactivity or interference between Human COL1 Alpha 1 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide. |
| Function | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Protein Name | Collagen Alpha-1(I ChainAlpha-1 Type I Collagen |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-198933Reactome: R-HSA-2022090Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3000480Reactome: R-HSA-430116Reactome: R-HSA-75892Reactome: R-HSA-76009Reactome: R-HSA-8874081Reactome: R-HSA-8940973Reactome: R-HSA-8948216Reactome: R-HSA-9845619Reactome: R-HSA-9845620Reactome: R-HSA-9845621Reactome: R-HSA-9845622Reactome: R-HSA-9846298 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative ELISA Names | Collagen Alpha-1(I Chain ELISA kitAlpha-1 Type I Collagen ELISA kitCOL1A1 ELISA kit |
| Specificity | This kit recognizes Human COL1α1 in samples. No significant cross-reactivity or interference between Human COL1α1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-97 | 92 |
| EDTA plasma (n=8) | 88-102 | 94 |
| Cell culture media (n=8) | 90-101 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.00 | 3.05 | 8.08 | 1.00 | 2.83 | 8.21 |
| Standard deviation | 0.06 | 0.16 | 0.38 | 0.07 | 0.16 | 0.43 |
| CV (%) | 6.00 | 5.25 | 4.70 | 7.00 | 5.65 | 5.24 |