| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human COL15A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
COL15A1 |
| Gene ID: |
1306 |
| Uniprot ID: |
COFA1_HUMAN |
| Specificity: |
This kit recognizes Human COL15 in samples. No significant cross-reactivity or interference between Human COL15 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Expressed predominantly in internal organs such as adrenal gland, pancreas and kidney. |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-glycosylated.with core 1 or possibly core 8 glycans. Contains chondroitin sulfate. |
| Function | Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle. Restin potently inhibits angiogenesis. |
| Protein Name | Collagen Alpha-1(Xv Chain Cleaved Into - RestinEndostatin-XvRelated To EndostatinRestin-I - Restin-2Restin-Ii - Restin-3Restin-Iii - Restin-4Restin-Iv |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1650814Reactome: R-HSA-2022090Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Collagen Alpha-1(Xv Chain Cleaved Into - Restin CLIA kitEndostatin-Xv CLIA kitRelated To Endostatin CLIA kitRestin-I - Restin-2 CLIA kitRestin-Ii - Restin-3 CLIA kitRestin-Iii - Restin-4 CLIA kitRestin-Iv CLIA kitCOL15A1 CLIA kit |
| Specificity | This kit recognizes Human COL15 in samples. No significant cross-reactivity or interference between Human COL15 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-106 | 99 |
| EDTA plasma(n=8) | 92-110 | 100 |
| Cell culture media(n=8) | 99-113 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 210.10 | 356.42 | 1457.03 | 204.53 | 371.55 | 1344.92 |
| Standard deviation | 25.53 | 40.70 | 107.53 | 24.20 | 39.79 | 117.01 |
| CV (%) | 12.15 | 11.42 | 7.38 | 11.83 | 10.71 | 8.70 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
