Human COL15A1 (Collagen alpha-1 (XV) chain) CLIA Kit for Quantitative Detection

Name: Human COL15A1 (Collagen alpha-1 (XV) chain) CLIA Kit for Quantitative Detection
SKU: STJC0000399
Availability: InStock

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SPECIFICATIONS

STJC0000399

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Short Description	This human COL15A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	COL15A1
Gene ID	1306
Uniprot ID	COFA1_HUMAN
Specificity	This kit recognizes Human COL15 in samples. No significant cross-reactivity or interference between Human COL15 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Expressed predominantly in internal organs such as adrenal gland, pancreas and kidney.
Post Translational Modifications	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-glycosylated.with core 1 or possibly core 8 glycans. Contains chondroitin sulfate.
Function	Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle. Restin potently inhibits angiogenesis.
Protein Name	Collagen Alpha-1(Xv Chain Cleaved Into - Restin Endostatin-Xv Related To Endostatin Restin-I - Restin-2 Restin-Ii - Restin-3 Restin-Iii - Restin-4 Restin-Iv
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1650814 Reactome: R-HSA-2022090 Reactome: R-HSA-8948216
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative CLIA Names	Collagen Alpha-1(Xv Chain Cleaved Into - Restin CLIA kit Endostatin-Xv CLIA kit Related To Endostatin CLIA kit Restin-I - Restin-2 CLIA kit Restin-Ii - Restin-3 CLIA kit Restin-Iii - Restin-4 CLIA kit Restin-Iv CLIA kit COL15A1 CLIA kit
Specificity	This kit recognizes Human COL15 in samples. No significant cross-reactivity or interference between Human COL15 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	210.10	356.42	1457.03	204.53	371.55	1344.92
Standard deviation	25.53	40.70	107.53	24.20	39.79	117.01
CV (%)	12.15	11.42	7.38	11.83	10.71	8.70