| Short Description : | Recombinant-Human Coagulation factor III/CD142-C-His protein was developed in hek293 cells using the region Gly34-Glu251. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | 1.Measured by its binding ability in a functional ELISA. Immobilized Human CD142 at 1 Mu g/mL (100 Mu L/well) can bind Human Tissue Factor Rabbit mAb with a linear range of 0.98-61.71 ng/mL.|2.Measured by its ability to activate Coagulation Factor VI |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | F3 |
| Gene ID: | 2152 |
| Uniprot ID: | TF_HUMAN |
| Immunogen: | Recombinant Human Coagulation factor III/CD142 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly34-Glu251) of human Coagulation Factor III/Tissue Factor/CD142 (Accession #NP_001984.1) fused with |
| Immunogen Region: | Gly34-Glu251 |
| Immunogen Sequence: | GTTNTVAAYNLTWKSTNFKT ILEWEPKPVNQVYTVQISTK SGDWKSKCFYTTDTECDLTD EIVKDVKQTYLARVFSYPAG NVESTGSAGEPLYENSPEFT PYLETNLGQPTIQSFEQVGT KVNVTVEDERTLVRRNNTFL SLRDVFGKDLIYTLYYWKSS SSGKKTAKTNTNEFLIDVDK GENYCFSVQAVIPSRTVNRK STDSPVECMGQEKGEFRE |
| Tissue Specificity | Lung, placenta and pancreas. |
| Function | Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. |
| Protein Name | Tissue FactorTfCoagulation Factor IiiThromboplastinCd Antigen Cd142 |
| Database Links | Reactome: R-HSA-140834Reactome: R-HSA-9031628 |
| Cellular Localisation | Isoform 1: MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative Protein Names | Tissue Factor proteinTf proteinCoagulation Factor Iii proteinThromboplastin proteinCd Antigen Cd142 proteinF3 protein |
Information sourced from Uniprot.org
