Human CMA1 (Chymase 1, Mast Cell) Sandwich ELISA Kit (STJE0019811)

Name: Human CMA1 (Chymase 1, Mast Cell) Sandwich ELISA Kit (STJE0019811)
SKU: STJE0019811
Availability: InStock

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SPECIFICATIONS

STJE0019811

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Short Description	This CMA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	46.88pg/mL
Detection Limit	78.13~5000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	CMA1
Gene ID	1215
Uniprot ID	CMA1_HUMAN
Specificity	This kit recognizes Human CMA1 in samples. No significant crossreactivity or interference between Human CMA1 and analogues was observed.
Sample Type	serum, plasma and other biological fluids

Tissue Specificity	Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Protein Name	Chymase Alpha-Chymase Mast Cell Protease I
Database Links	Reactome: R-HSA-1433557 Reactome: R-HSA-1592389 Reactome: R-HSA-2022377
Cellular Localisation	Secreted Cytoplasmic Granule Mast Cell Granules
Alternative ELISA Names	Chymase ELISA kit Alpha-Chymase ELISA kit Mast Cell Protease I ELISA kit CMA1 ELISA kit CYH ELISA kit CYM ELISA kit
Specificity	This kit recognizes Human CMA1 in samples. No significant cross-reactivity or interference between Human CMA1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	276.09	648.72	2043.14	290.74	605.11	2165.00
Standard deviation	17.45	36.98	68.45	15.67	28.32	105.65
CV (%)	6.32	5.70	3.35	5.39	4.68	4.88