This ChT Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.057ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Expressed in putamen, spinal cord and medulla. Expressed in cholinergic neurons.
Post Translational Modifications
Phosphorylated.
Function
High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity.
Protein Name
High Affinity Choline Transporter 1Hcht1Hemicholinium-3-Sensitive Choline TransporterChtSolute Carrier Family 5 Member 7
Presynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionAxonEarly Endosome MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneLocalized At The Neuromuscular JunctionLocalization At The Plasma Membrane Is Transient Due To The Rapid Endocytosis Of Slc5a7/Cht1 Via The Clathrin-Mediated PathwayWhere It Localizes To Early EndosomesAlso Localized To Synaptic Vesicles Where It Is Likely Mobilized To The Cell Surface By Exocytosis
Alternative ELISA Names
High Affinity Choline Transporter 1 ELISA kitHcht1 ELISA kitHemicholinium-3-Sensitive Choline Transporter ELISA kitCht ELISA kitSolute Carrier Family 5 Member 7 ELISA kitSLC5A7 ELISA kitCHT1 ELISA kit
