Human CHST4 protein (Recombinant-Active) (N-His) (STJP012206)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human CHST4-N-His protein was developed from mammalian cells and has a target region of N-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to transfer sulfate from PAPS to N-acetyl-D-glucosamine. The specific activity is >150 pmol/min/Mu g, as measured under the described conditions. |
Target Information
| Gene Symbol | CHST4 |
| Gene ID | 10164 |
| Uniprot ID | CHST4_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | His29-His386 |
Additional Info
| Function | Sulfotransferase involved in SELL/L-selectin ligand biosynthesis pathway. Catalyzes the transfer of the sulfate group from 3'-phospho-5'-adenylyl sulfate (PAPS) onto the hydroxyl group at C-6 position of the non-reducing N-acetylglucosamine (GlcNAc) residue within O-linked mucin-type glycans. Contributes to generate sialyl 6-sulfo Lewis X determinant (also known as MECA-79 epitope) for SELL recognition, a prerequisite for continuous lymphocyte homing into peripheral lymph nodes and antigen immune surveillance. Transfers the sulfate group primarily on core 2 GlcNAcbeta1-6(Galbeta1-3)GalNAcalphaSer/Thr and extended core 1 GlcNAcbeta1-3Galbeta1-3GalNAcalphaSer/Thr based O-linked glycans on CD34 and GLYCAM1 peripheral node addressins (PNAds) expressed on the lumenal side of high endothelial venules (HEVs). The recognition of PNAds by SELL initiates a multistep process comprising tethering and rolling of blood lymphocytes on HEVs against the blood flow, followed by chemokine signaling, integrin-mediated lymphocyte adhesion onto endothelial cells and lymphocyte transendothelial migration. Modulates rolling velocity and differential T and B lymphocyte recruitment into peripheral lymph nodes, with a major role in B lymphocyte homing. Might be redundant in sulfation of MADCAM1 and lymphocyte trafficking to mesenteric lymph nodes. Can also sulfonate core 3 GlcNAcbeta1-3GalNAc-R based glycans as well as GlcNAcbeta1-3Galbeta1-Glc, GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharides, which might be ectopically expressed during tumorigenesis. |
| Protein Name | Carbohydrate Sulfotransferase 4Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3Gst-3High Endothelial Cells N-Acetylglucosamine 6-O-SulfotransferaseHec-Glcnac6stL-Selectin Ligand SulfotransferaseLsstN-Acetylglucosamine 6-O-Sulfotransferase 2Glcnac6st-2Gn6st-2 |
| Database Links | Reactome: R-HSA-913709 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Protein Names | Carbohydrate Sulfotransferase 4 proteinGalactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3 proteinGst-3 proteinHigh Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase proteinHec-Glcnac6st proteinL-Selectin Ligand Sulfotransferase proteinLsst proteinN-Acetylglucosamine 6-O-Sulfotransferase 2 proteinGlcnac6st-2 proteinGn6st-2 proteinCHST4 protein |
Information sourced from Uniprot.org