Human CHMP2A protein (Recombinant) (His-Tag) (STJP016512)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human CHMP2A-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 30% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHMP2A |
| Gene ID | 27243 |
| Uniprot ID | CHM2A_HUMAN |
| Accession Number | NP_055268.1 |
| Immunogen | Human |
| Immunogen Region | 1-222aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | ISGylated in a CHMP5-dependent manner. Isgylation weakens and inhibits its interactions with VPS4A and VTA1 respectively. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. (Microbial infection) The ESCRT machinery functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Involved in HIV-1 p6- and p9-dependent virus release. |
| Protein Name | Charged Multivesicular Body Protein 2aChromatin-Modifying Protein 2aChmp2aPutative Breast Adenocarcinoma Marker Bc-2Vacuolar Protein Sorting-Associated Protein 2-1Vps2-1Hvps2-1 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-1632852Reactome: R-HSA-432720Reactome: R-HSA-5620971Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710Reactome: R-HSA-9668328Reactome: R-HSA-9679504Reactome: R-HSA-9694676 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideNucleus EnvelopeLocalizes To The Midbody Of Dividing CellsLocalized In Two Distinct Rings On Either Side Of The Fleming BodyLocalizes To The Reforming Nuclear Envelope On Chromatin Disks During Late Anaphase |
| Alternative Protein Names | Charged Multivesicular Body Protein 2a proteinChromatin-Modifying Protein 2a proteinChmp2a proteinPutative Breast Adenocarcinoma Marker Bc-2 proteinVacuolar Protein Sorting-Associated Protein 2-1 proteinVps2-1 proteinHvps2-1 proteinCHMP2A proteinBC2 proteinCHMP2 protein |
Information sourced from Uniprot.org