Human CHKA protein (Recombinant) (N-His) (STJP010900)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CHKA-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CHKA |
| Gene ID | 1119 |
| Uniprot ID | CHKA_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp138-Leu376 |
Additional Info
| Function | Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis. Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis. Has higher activity with choline. May contribute to tumor cell growth. Isoform 1: This isoform plays a key role in lipolysis of lipid droplets following glucose deprivation. In response to glucose deprivation, phosphorylated by AMPK, promoting localization to lipid droplets. Phosphorylation is followed by acetylation by KAT5, leading to dissociation of the homodimer into a monomer. Monomeric CHKA isoform 1 is converted into a tyrosine-protein kinase, which phosphorylates lipid droplet structural proteins PLIN2 and PLIN3, leading to lipolysis of lipid droplets. |
| Protein Name | Choline Kinase AlphaCkChetk-AlphaEthanolamine KinaseEk |
| Database Links | Reactome: R-HSA-1483191Reactome: R-HSA-1483213 |
| Cellular Localisation | CytoplasmCytosolIsoform 1: Lipid DropletIsoform 1 Localizes To Lipid Droplets Following Phosphorylation By Ampk |
| Alternative Protein Names | Choline Kinase Alpha proteinCk proteinChetk-Alpha proteinEthanolamine Kinase proteinEk proteinCHKA proteinCHK proteinCKI protein |
Information sourced from Uniprot.org