Human Chitinase-1/CHIT1 protein (Recombinant) (C-His) (STJP001019)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Chitinase-1/CHIT1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala22-Asn466) of human CHIT1 (Accession #NP_003456) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Chitinase-1/CHIT1-C-His protein was developed in hek293 cells using the region Ala22-Asn466. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave 4-Methylumbelliferyl-beta-D-N, N NA, N”-triacetylchitotriose (4-MU-TACT). The specific activity is >43571 pmol/min/Mu g. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CHIT1 |
| Gene ID | 1118 |
| Uniprot ID | CHIT1_HUMAN |
| Immunogen | Recombinant Human Chitinase-1/CHIT1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala22-Asn466) of human CHIT1 (Accession #NP_003456) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Ala22-Asn466 |
| Immunogen Sequence | AKLVCYFTNWAQYRQGEARF LPKDLDPSLCTHLIYAFAGM TNHQLSTTEWNDETLYQEFN GLKKMNPKLKTLLAIGGWNF STQKFTDMVATANNRQTFVN SAIRFLRKYSFDGLDLDWEY PGSQGSPAVDKERFTTLVQD LANAFQQEAQTSGKERLLLS AAVPAGQTYVDAGYEVDKIA QNLDFVNLMAYDFHGSWEKV TGHNSPLYKRQEESGAAASL NVDAAVQQWLQKGTPASKL |
Additional Info
| Tissue Specificity | Detected in spleen. Secreted by cultured macrophages. |
| Function | Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. |
| Protein Name | Chitotriosidase-1Chitinase-1 |
| Database Links | Reactome: R-HSA-189085Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedLysosomeA Small Proportion Is Lysosomal |
| Alternative Protein Names | Chitotriosidase-1 proteinChitinase-1 proteinCHIT1 protein |
Information sourced from Uniprot.org