Human CFD (Complement Factor D) Antibody Pair (STJAP00380)
General Information
| Short Description | This CFD antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | CFD |
| Gene ID | 1675 |
| Uniprot ID | CFAD_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human CFD protien.Detection Immunogen: Recombinant Human CFD protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway. |
| Protein Name | Complement Factor DAdipsinC3 Convertase ActivatorProperdin Factor D |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-173736Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Complement Factor D antibody pairAdipsin antibody pairC3 Convertase Activator antibody pairProperdin Factor D antibody pairCFD antibody pairDF antibody pairPFD antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CFD (Complement Factor D) Antibody Pair (STJAP00380)
This antibody pair detects human Complement Factor D (CFD), corresponding to UniProt entry P00746. Reactivity is confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated, both at 0.5 mg/mL. Both are goat-derived and purified by antigen affinity.
This pair is validated for use in ELISA. It has not been described as validated for other assay formats based on the available product information.
This pair is suitable for use with serum, plasma, and other biological fluids. All applications are for research use only and are not intended for diagnostic or therapeutic purposes.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20 degrees Celsius for long-term storage, and freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant human CFD protein. Both antibodies were subsequently purified by antigen affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.