Human CENPF protein (Recombinant) (N-His) (STJP004816)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CENPF-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CENPF |
| Gene ID | 1063 |
| Uniprot ID | CENPF_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gln436-Lys637 |
Additional Info
| Function | Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. |
| Protein Name | Centromere Protein FCenp-FAh AntigenKinetochore Protein CenpfMitosin |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-156711Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleus MatrixChromosomeCentromereKinetochoreCytoskeletonSpindleRelocalizes To The Kinetochore/Centromere (Coronal Surface Of The Outer Plate) And The Spindle During MitosisObserved In Nucleus During Interphase But Not In The NucleolusAt Metaphase Becomes Localized To Areas Including Kinetochore And Mitotic Apparatus As Well As CytoplasmBy TelophaseIs Concentrated Within The Intracellular Bridge At Either Side Of The Mid-Body |
| Alternative Protein Names | Centromere Protein F proteinCenp-F proteinAh Antigen proteinKinetochore Protein Cenpf proteinMitosin proteinCENPF protein |
Information sourced from Uniprot.org