Human CDKN1A protein (Recombinant) (N-His) (STJP004663)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CDKN1A-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CDKN1A |
| Gene ID | 1026 |
| Uniprot ID | CDN1A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Pro164 |
Additional Info
| Post Translational Modifications | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
| Function | Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes. |
| Protein Name | Cyclin-Dependent Kinase Inhibitor 1Cdk-Interacting Protein 1Melanoma Differentiation-Associated Protein 6Mda-6P21 |
| Database Links | Reactome: R-HSA-187577Reactome: R-HSA-198323Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-5674400Reactome: R-HSA-6785807Reactome: R-HSA-6804116Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69563Reactome: R-HSA-69656Reactome: R-HSA-69895Reactome: R-HSA-8852276Reactome: R-HSA-8866911Reactome: R-HSA-8878166Reactome: R-HSA-8941855Reactome: R-HSA-8951664Reactome: R-HSA-9616222Reactome: R-HSA-9617828Reactome: R-HSA-9661069Reactome: R-HSA-9702518Reactome: R-HSA-9703465Reactome: R-HSA-9725370Reactome: R-HSA-9755511Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Protein Names | Cyclin-Dependent Kinase Inhibitor 1 proteinCdk-Interacting Protein 1 proteinMelanoma Differentiation-Associated Protein 6 proteinMda-6 proteinP21 proteinCDKN1A proteinCAP20 proteinCDKN1 proteinCIP1 proteinMDA6 proteinPIC1 proteinSDI1 proteinWAF1 protein |
Information sourced from Uniprot.org