This human CDK6 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications
CLIA
Reactivity
Human
Sensitivity
18.75pg/mL
Detection Limit
31.25~2000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human CDK6 in samples. No significant cross-reactivity or interference between Human CDK6 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Tissue Specificity
Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.
Post Translational Modifications
Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.
Function
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation.promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation.prevents cell proliferation and negatively regulates cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases.
Protein Name
Cyclin-Dependent Kinase 6Cell Division Protein Kinase 6Serine/Threonine-Protein Kinase Plstire
CytoplasmNucleusCell ProjectionRuffleCytoskeletonMicrotubule Organizing CenterCentrosomeLocalized To The Ruffling Edge Of Spreading FibroblastsKinase Activity Only In NucleusLocalized To The Cytosol Of Neurons And Showed Prominent Staining Around Either Side Of The NucleusPresent In The Cytosol And In The Nucleus In Interphase Cells And At The Centrosome During Mitosis From Prophase To Telophase
