Human CD68 (CD68) Antibody Pair (STJAP00768)
General Information
| Short Description | This CD68 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R74-8I-8/R43-7B-7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CD68 |
| Gene ID | 968 |
| Uniprot ID | CD68_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. |
| Protein Name | MacrosialinGp110Cd Antigen Cd68 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Short: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Long: Endosome MembraneLysosome Membrane |
| Alternative Antibody Pair Names | Macrosialin antibody pairGp110 antibody pairCd Antigen Cd68 antibody pairCD68 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CD68 (CD68) Antibody Pair (STJAP00768)
This antibody pair is designed to detect human CD68 (UniProt P34810) and has confirmed reactivity against human samples only. Reactivity in other species has not been specified for this product.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs at a concentration of 1 mg/mL, formulated in 0.01M PBS at pH 7.2. Clone IDs are R74-8I-8 and R43-7B-7 for the two components of the pair.
Working dilutions for both antibodies are to be determined by the end user and are not pre-defined in the product specifications. Titration experiments are recommended to establish optimal concentrations for each specific assay setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Both antibodies in this pair were raised in rabbit against a recombinant protein immunogen corresponding to human CD68. No further details regarding the specific immunogen region are provided in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.