Human CD5 protein (Recombinant) (C-His) (STJP000933)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CD5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg25-Asn371) of human CD5 (Accession #NP_055022.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CD5-C-His protein was developed in hek293 cells using the region Arg25-Asn371. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD5 |
| Gene ID | 921 |
| Uniprot ID | CD5_HUMAN |
| Immunogen | Recombinant Human CD5 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg25-Asn371) of human CD5 (Accession #NP_055022.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Arg25-Asn371 |
| Immunogen Sequence | RLSWYDPDFQARLTRSNSKC QGQLEVYLKDGWHMVCSQSW GRSSKQWEDPSQASKVCQRL NCGVPLSLGPFLVTYTPQSS IICYGQLGSFSNCSHSRNDM CHSLGLTCLEPQKTTPPTTR PPPTTTPEPTAPPRLQLVAQ SGGQHCAGVVEFYSGSLGGT ISYEAQDKTQDLENFLCNNL QCGSFLKHLPETEAGRAQDP GEPREHQPLPIQWKIQNSSC TSLEHCFRKIKPQKSGRVL |
Additional Info
| Post Translational Modifications | Phosphorylated on serine, threonine and tyrosine residues following TCR stimulation. Phosphorylated by LCK on Tyr-453 and Tyr-487 upon TCR engagement. |
| Function | Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production. |
| Protein Name | T-Cell Surface Glycoprotein Cd5Lymphocyte Antigen T1/Leu-1Cd Antigen Cd5 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Cell Surface Glycoprotein Cd5 proteinLymphocyte Antigen T1/Leu-1 proteinCd Antigen Cd5 proteinCD5 proteinLEU1 protein |
Information sourced from Uniprot.org