Human CD44 protein (Recombinant) (C-hFc) (STJP019977)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CD44 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln21-Pro220 & Gln386-Ala427) of Human CD44 (Accession #P16070-1) fused with a C-hFc tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CD44-C-hFc protein was developed in hek293 cells using the region Gln21-Pro220 & Gln386-Ala427. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD44 |
| Gene ID | 960 |
| Uniprot ID | CD44_HUMAN |
| Immunogen | Recombinant Human CD44 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln21-Pro220 & Gln386-Ala427) of Human CD44 (Accession #P16070-1) fused with a C-hFc tag at the C-terminus. |
| Immunogen Region | Gln21-Pro220 & Gln386-Ala427 |
| Immunogen Sequence | QIDLNITCRFAGVFHVEKNG RYSISRTEAADLCKAFNSTL PTMAQMEKALSIGFETCRYG FIEGHVVIPRIHPNSICAAN NTGVYILTSNTSQYDTYCFN ASAPPEEDCTSVTDLPNAFD GPITITIVNRDGTRYVQKGE YRTNPEDIYPSNPTDDDVSS GSSSERSSTSGGYIFYTFST VHPIPDEDSPWITDSTDRIP QATPSSTTEETATQKEQWFG NRWHEGYRQTPKEDSHSTT |
Additional Info
| Tissue Specificity | Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. |
| Post Translational Modifications | Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. |
| Function | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
| Protein Name | Cd44 AntigenCdw44EpicanExtracellular Matrix Receptor IiiEcmr-IiiGp90 Lymphocyte Homing/Adhesion ReceptorHutch-IHeparan Sulfate ProteoglycanHermes AntigenHyaluronate ReceptorPhagocytic Glycoprotein 1Pgp-1Phagocytic Glycoprotein IPgp-ICd Antigen Cd44 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2160916Reactome: R-HSA-6798695Reactome: R-HSA-877300 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionMicrovillusSecretedColocalizes With Actin In Membrane Protrusions At Wounding EdgesCo-Localizes With RdxEzr And Msn In MicrovilliLocalizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains |
| Alternative Protein Names | Cd44 Antigen proteinCdw44 proteinEpican proteinExtracellular Matrix Receptor Iii proteinEcmr-Iii proteinGp90 Lymphocyte Homing/Adhesion Receptor proteinHutch-I proteinHeparan Sulfate Proteoglycan proteinHermes Antigen proteinHyaluronate Receptor proteinPhagocytic Glycoprotein 1 proteinPgp-1 proteinPhagocytic Glycoprotein I proteinPgp-I proteinCd Antigen Cd44 proteinCD44 proteinLHR proteinMDU2 proteinMDU3 proteinMIC4 protein |
Information sourced from Uniprot.org