Human CD43 protein (Recombinant) (His-Tag) (STJP018413)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human CD43-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPN |
| Gene ID | 6693 |
| Uniprot ID | LEUK_HUMAN |
| Accession Number | NP_003114.1 |
| Immunogen | Human |
| Immunogen Region | 20-253aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. |
| Post Translational Modifications | Glycosylated.has a high content of sialic acid and O-linked carbohydrate structures. Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes. Has a high content of sialic acid and O-linked carbohydrate structures. Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. CD43 cytoplasmic tail: Sumoylated. |
| Function | Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1. CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival. |
| Protein Name | LeukosialinGpl115GalactoglycoproteinGalgpLeukocyte SialoglycoproteinSialophorinCd Antigen Cd43 Cleaved Into - Cd43 Cytoplasmic TailCd43-CtCd43ct |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-210991 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCell ProjectionMicrovillusUropodiumLocalizes To The Uropodium And Microvilli Via Its Interaction With Erm Proteins (EzrRdx And Msn)Cd43 Cytoplasmic Tail: NucleusNucleusPml BodyThe Sumoylated Form Localizes To The Pml Body |
| Alternative Protein Names | Leukosialin proteinGpl115 proteinGalactoglycoproteinGalgp proteinLeukocyte SialoglycoproteinSialophorin proteinCd Antigen Cd43 Cleaved Into - Cd43 Cytoplasmic Tail proteinCd43-Ct proteinCd43ct proteinSPN proteinCD43 protein |
Information sourced from Uniprot.org