Human CD40L (Cluster Of Differentiation 40 Ligand) Sandwich ELISA Kit Ready-To-Use Kit (STJE0014189)
SPECIFICATIONS
General Information
| Short Description | This CD40L Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.051ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | CD40LG |
| Gene ID | 959 |
| Uniprot ID | CD40L_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Specifically expressed on activated CD4+ T-lymphocytes. |
| Post Translational Modifications | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
| Function | Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3.both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling. |
| Protein Name | Cd40 LigandCd40-LT-Cell Antigen Gp39Tnf-Related Activation ProteinTrapTumor Necrosis Factor Ligand Superfamily Member 5Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble FormScd40l |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-5668541Reactome: R-HSA-5676594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinCell SurfaceCd40 LigandSoluble Form: SecretedRelease Of Soluble Cd40l From Platelets Is Partially Regulated By Gp Iib/IiiaActin PolymerizationAnd A Matrix Metalloproteinases (Mmp) Inhibitor-Sensitive Pathway |
| Alternative ELISA Names | Cd40 Ligand ELISA kitCd40-L ELISA kitT-Cell Antigen Gp39 ELISA kitTnf-Related Activation Protein ELISA kitTrap ELISA kitTumor Necrosis Factor Ligand Superfamily Member 5 ELISA kitCd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form ELISA kitScd40l ELISA kitCD40LG ELISA kitCD40L ELISA kitTNFSF5 ELISA kitTRAP ELISA kit |
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Information sourced from Uniprot.org