Human CD40 Ligand/TNFSF5 Trimer protein (Recombinant) (N-His&Flag) (STJP019979)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human TNFSF5/CD40 ligand/CD154 Trimer Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met113-Leu261) of Human TNFSF5/CD40 ligand/CD154 Trimer (Accession #P29965) fused with a N-His&Fla
General Information
| Short Description | Recombinant-Human CD40 Ligand/TNFSF5 Trimer-N-His&Flag protein was developed in hek293 cells using the region Met113-Leu261. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD40LG |
| Gene ID | 959 |
| Uniprot ID | CD40L_HUMAN |
| Immunogen | Recombinant Human TNFSF5/CD40 ligand/CD154 Trimer Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met113-Leu261) of Human TNFSF5/CD40 ligand/CD154 Trimer (Accession #P29965) fused with a N-His&Fla |
| Immunogen Region | Met113-Leu261 |
| Immunogen Sequence | MIETYNQTSPRSAATGLPIS MKIFMYLLTVFLITQMIGSA LFAVYLHRRLDKIEDERNLH EDFVFMKTIQRCNTGERSLS LLNCEEIKSQFEGFVKDIML NKEETKKENSFEMQKGDQNP QIAAHVISEASSKTTSVLQW AEKGYYTMSNNLVTLENGKQ LTVKRQGLYYIYAQVTFCSN REASSQAPFIASLCLKSPGR FERILLRAANTHSSAKPCGQ QSIHLGGVFELQPGASVFV |
Additional Info
| Tissue Specificity | Specifically expressed on activated CD4+ T-lymphocytes. |
| Post Translational Modifications | The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. |
| Function | Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3.both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling. |
| Protein Name | Cd40 LigandCd40-LT-Cell Antigen Gp39Tnf-Related Activation ProteinTrapTumor Necrosis Factor Ligand Superfamily Member 5Cd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble FormScd40l |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-5668541Reactome: R-HSA-5676594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinCell SurfaceCd40 LigandSoluble Form: SecretedRelease Of Soluble Cd40l From Platelets Is Partially Regulated By Gp Iib/IiiaActin PolymerizationAnd A Matrix Metalloproteinases (Mmp) Inhibitor-Sensitive Pathway |
| Alternative Protein Names | Cd40 Ligand proteinCd40-L proteinT-Cell Antigen Gp39 proteinTnf-Related Activation Protein proteinTrap proteinTumor Necrosis Factor Ligand Superfamily Member 5 proteinCd Antigen Cd154 Cleaved Into - Cd40 Ligand - Membrane Form - Cd40 Ligand - Soluble Form proteinScd40l proteinCD40LG proteinCD40L proteinTNFSF5 proteinTRAP protein |
Information sourced from Uniprot.org