Human CD32a (FcgRIIa) protein (Recombinant) {Biotin} (STJP000197)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenOptimized DNA sequence encoding Human extracellular domain of human CD32aincluding a C-terminal 6His tag was expressed in HEK293 cells. CD32a was further labled by Biotin for detection by avidin conjugated secondary probes. NA
General Information
| Short Description | Recombinant-Human CD32a (FcgRIIa)-protein was developed from hek293. For use in research applications. |
| Applications | ELISA/WB |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. |
| Formulation | Lyophilised 0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
Target Information
| Gene Symbol | FCGR2A |
| Gene ID | 2212 |
| Uniprot ID | FCG2A_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human extracellular domain of human CD32aincluding a C-terminal 6His tag was expressed in HEK293 cells. CD32a was further labled by Biotin for detection by avidin conjugated secondary probes. NA |
| Immunogen Region | ECD |
Additional Info
| Tissue Specificity | Found on monocytes, neutrophils and eosinophil platelets. |
| Post Translational Modifications | Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. |
| Function | Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. |
| Protein Name | Low Affinity Immunoglobulin Gamma Fc Region Receptor Ii-AIgg Fc Receptor Ii-ACdw32Fc-Gamma Rii-AFc-Gamma-RiiaFcrii-ACd Antigen Cd32 |
| Database Links | Reactome: R-HSA-2029481Reactome: R-HSA-2029482Reactome: R-HSA-2029485Reactome: R-HSA-6798695Reactome: R-HSA-9664323 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Low Affinity Immunoglobulin Gamma Fc Region Receptor Ii-A proteinIgg Fc Receptor Ii-A proteinCdw32 proteinFc-Gamma Rii-A proteinFc-Gamma-Riia proteinFcrii-A proteinCd Antigen Cd32 proteinFCGR2A proteinCD32 proteinFCG2 proteinFCGR2A1 proteinIGFR2 protein |
Information sourced from Uniprot.org