• Biotinylated Human ITGAV&ITGB5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human ITGAV&ITGB5 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-alpha V Antibody, hFc Tag at 1 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB5, His Tag with the EC50 of 0. 29ug/ml determined by ELISA.

Human CD3 Epsilon Delta heterodimer protein (Recombinant) (STJP000253)

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STJP000253

£228.50 - £3,640.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human CD3 Epsilon Delta heterodimer-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by the ability of immobilized recombinant human CD3 epsilon-CD3 delta heterodimer to bind Human OKT3 with a linear range of 5-20 ng/ml NA
Gene Symbol: CD3E
Gene ID: 916
Uniprot ID: CD3E_HUMAN
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of Human CD3 Epsilon including a flag tag was co-expressed with aDNA sequence encoding the extracellular domain of CD3 Delta including a C-terminal His tag, were expressed in HEK293 cells. NA
Post Translational Modifications Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
Protein Name T-Cell Surface Glycoprotein Cd3 Epsilon Chain
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain
Cd Antigen Cd3e
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names T-Cell Surface Glycoprotein Cd3 Epsilon Chain protein
T-Cell Surface Antigen T3/Leu-4 Epsilon Chain protein
Cd Antigen Cd3e protein
CD3E protein
T3E protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance