| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
1.Measured by its binding ability in a functional ELISA. Immobilized Human CD28 Protein at 1 Mu g/mL (100 Mu L/well) can bind CD28 Rabbit mAb with a linear range of 0.06-1.04 ng/mL. |
| Gene Symbol: |
CD28 |
| Gene ID: |
940 |
| Uniprot ID: |
CD28_HUMAN |
| Immunogen Region: |
Asn19-Pro152 |
| Immunogen: |
Recombinant Human CD28 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asn19-Pro152) of human CD28/TP44 (Accession #NP_006130.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
NKILVKQSPMLVAYDNAVNL SCKYSYNLFSREFRASLHKG LDSAVEVCVVYGNYSQQLQV YSKTGFNCDGKLGNESVTFY LQNLYVNQTDIYFCKIEVMY PPPYLDNEKSNGTIIHVKGK HLCPSPLFPGPSKP |
| Tissue Specificity | Expressed in T-cells and plasma cells, but not in less mature B-cells. |
| Post Translational Modifications | Phosphorylated by LCK. Dephosphorylated by PTPN11. Isoform 3: Tyrosine phosphorylated induced by CD40LG. |
| Function | Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells. Isoform 3: Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. |
| Protein Name | T-Cell-Specific Surface Glycoprotein Cd28Tp44Cd Antigen Cd28 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-164939Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell Surface |
| Alternative Protein Names | T-Cell-Specific Surface Glycoprotein Cd28 proteinTp44 proteinCd Antigen Cd28 proteinCD28 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
