Human CD279/PDCD1/PD1 protein (Recombinant-Active) (C-His) (STJP012678)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human CD279/PDCD1/PD1-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 7.5. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8ยฐC for one week. Store at-20 to-80ยฐC for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/ยตg as determined by LAL test. |
Target Information
| Gene Symbol | PDCD1 |
| Gene ID | 5133 |
| Uniprot ID | PDCD1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Pro21-Gln167 |
Additional Info
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Hpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-HSA-389948Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Programmed Cell Death Protein 1 proteinProtein Pd-1 proteinHpd-1 proteinCd Antigen Cd279 proteinPDCD1 proteinPD1 protein |
Information sourced from Uniprot.org