Human CD257/TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) Sandwich ELISA Kit (STJE0001915)

Name: Human CD257/TNFSF13B (Tumor necrosis factor ligand superfamily member 13B) Sandwich ELISA Kit (STJE0001915)
SKU: STJE0001915
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0001915

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General Information

Short Description	This CD257/TNFSF13B Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	37.5pg/mL
Detection Limit	62.5~4000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	TNFSF13B
Gene ID	10673
Uniprot ID	TN13B_HUMAN
Specificity	This kit recognizes Human BAFF/CD257 in samples. No significant cross-reactivity or interference between Human BAFF/CD257 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated.
Function	Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 13b B Lymphocyte Stimulator Blys B-Cell-Activating Factor Baff Dendritic Cell-Derived Tnf-Like Molecule Tnf- And Apol-Related Leukocyte Expressed Ligand 1 Tall-1 Cd Antigen Cd257 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 13b - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 13b - Soluble Form
Database Links	Reactome: R-HSA-5668541 Reactome: R-HSA-5669034 Reactome: R-HSA-5676594
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Ligand Superfamily Member 13b Soluble Form: Secreted
Alternative ELISA Names	Tumor Necrosis Factor Ligand Superfamily Member 13b ELISA kit B Lymphocyte Stimulator ELISA kit Blys ELISA kit B-Cell-Activating Factor ELISA kit Baff ELISA kit Dendritic Cell-Derived Tnf-Like Molecule ELISA kit Tnf- And Apol-Related Leukocyte Expressed Ligand 1 ELISA kit Tall-1 ELISA kit Cd Antigen Cd257 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 13b - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 13b - Soluble Form ELISA kit TNFSF13B ELISA kit BAFF ELISA kit BLYS ELISA kit TALL1 ELISA kit TNFSF20 ELISA kit ZTNF4 ELISA kit UNQ401 ELISA kit PRO738 ELISA kit
Specificity	This kit recognizes Human BAFF/CD257 in samples. No significant cross-reactivity or interference between Human BAFF/CD257 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-101	94
EDTA plasma (n=8)	86-100	91
Cell culture media (n=8)	90-105	96

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	185.79	600.16	1343.28	173.82	543.69	1425.24
Standard deviation	11.65	35.47	54.13	11.94	28.71	45.04
CV (%)	6.27	5.91	4.03	6.87	5.28	3.16

Citations

View product citations for antibody STJE0001915 on CiteAb