Human CD22 protein (Recombinant-Active) (C-hFc-His) (STJP011908)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human CD22-C-hFc-His protein was developed from mammalian cells and has a target region of C-hFc-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Immobilised human CD22 at 0.5 Mu g/mL (100 Mu L/well) can bind anti-CD22 antibody with a linear range of 0.01-1.28 ng/mL. |
Target Information
| Gene Symbol | CD22 |
| Gene ID | 933 |
| Uniprot ID | CD22_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp20-Arg687 |
Additional Info
| Post Translational Modifications | Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. Phosphorylated on tyrosine residues by LYN. |
| Function | Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow. Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration. Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling. If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced. |
| Protein Name | B-Cell Receptor Cd22B-Lymphocyte Cell Adhesion MoleculeBl-CamSialic Acid-Binding Ig-Like Lectin 2Siglec-2T-Cell Surface Antigen Leu-14Cd Antigen Cd22 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-5690714Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | B-Cell Receptor Cd22 proteinB-Lymphocyte Cell Adhesion Molecule proteinBl-Cam proteinSialic Acid-Binding Ig-Like Lectin 2 proteinSiglec-2 proteinT-Cell Surface Antigen Leu-14 proteinCd Antigen Cd22 proteinCD22 proteinSIGLEC2 protein |
Information sourced from Uniprot.org