Human CD163 protein (Recombinant) (N-His) (STJP005652)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CD163-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CD163 |
| Gene ID | 9332 |
| Uniprot ID | C163A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly46-Gly401 |
Additional Info
| Post Translational Modifications | A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. |
| Function | Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. |
| Protein Name | Scavenger Receptor Cysteine-Rich Type 1 Protein M130Hemoglobin Scavenger ReceptorCd Antigen Cd163 Cleaved Into - Soluble Cd163Scd163 |
| Database Links | Reactome: R-HSA-2168880Reactome: R-HSA-9662834 |
| Cellular Localisation | Soluble Cd163: SecretedCell MembraneSingle-Pass Type I Membrane ProteinIsoform 1 And Isoform 2 Show A Lower Surface Expression When Expressed In Cells |
| Alternative Protein Names | Scavenger Receptor Cysteine-Rich Type 1 Protein M130 proteinHemoglobin Scavenger Receptor proteinCd Antigen Cd163 Cleaved Into - Soluble Cd163 proteinScd163 proteinCD163 proteinM130 protein |
Information sourced from Uniprot.org