Human CD163 protein (Recombinant) (C-His&Avi) (STJP020149)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CD163 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser42-Ser1045) of Human CD163 fused with a His tag and Avi tag at the C-terminal.
General Information
| Short Description | Recombinant-Human CD163-C-His&Avi protein was developed in hek293 cells using the region Ser42-Ser1045. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CD163 |
| Gene ID | 9332 |
| Uniprot ID | C163A_HUMAN |
| Immunogen | Recombinant Human CD163 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser42-Ser1045) of Human CD163 fused with a His tag and Avi tag at the C-terminal. |
| Immunogen Region | Ser42-Ser1045 |
Additional Info
| Post Translational Modifications | A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. |
| Function | Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. |
| Protein Name | Scavenger Receptor Cysteine-Rich Type 1 Protein M130Hemoglobin Scavenger ReceptorCd Antigen Cd163 Cleaved Into - Soluble Cd163Scd163 |
| Database Links | Reactome: R-HSA-2168880Reactome: R-HSA-9662834 |
| Cellular Localisation | Soluble Cd163: SecretedCell MembraneSingle-Pass Type I Membrane ProteinIsoform 1 And Isoform 2 Show A Lower Surface Expression When Expressed In Cells |
| Alternative Protein Names | Scavenger Receptor Cysteine-Rich Type 1 Protein M130 proteinHemoglobin Scavenger Receptor proteinCd Antigen Cd163 Cleaved Into - Soluble Cd163 proteinScd163 proteinCD163 proteinM130 protein |
Information sourced from Uniprot.org