Human CD147 (CD147) Antibody Pair (STJAP00764)
General Information
| Short Description | This CD147 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R54-9C-6/R89-2G-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | BSG |
| Gene ID | 682 |
| Uniprot ID | BASI_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Isoform 1: Retina-specific. Expressed in retinal cone photoreceptors (at protein level). Isoform 2: Expressed in erythrocytes (at protein level). Highly expressed in melanoma cell lines (at protein level). Highly expressed in the heart, kidney, skeletal muscle and testis. Isoform 3: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus. Isoform 4: Highly expressed in the bone marrow, fetal liver, lung, testis and thymus. |
| Post Translational Modifications | Isoform 2: N-glycosylated. Isoform 3: N-glycosylated. Isoform 4: N-glycosylated. |
| Function | Isoform 1: Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes. Isoform 1: (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2. Isoform 2: Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells. Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane. Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling. Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells. Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells). Isoform 2: (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3. Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte. This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion. Isoform 2: (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA. Isoform 2: (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA. Isoform 2: (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection. Isoform 2: (Microbial infection) Acts as a receptor for measles virus. Isoform 2: (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells. |
| Protein Name | Basigin5f7Collagenase Stimulatory FactorExtracellular Matrix Metalloproteinase InducerEmmprinHepatoma-Associated AntigenHab18gLeukocyte Activation Antigen M6Ok Blood Group AntigenTumor Cell-Derived Collagenase Stimulatory FactorTcsfCd Antigen Cd147 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-210991Reactome: R-HSA-216083Reactome: R-HSA-433692Reactome: R-HSA-5619070Reactome: R-HSA-9749641 |
| Cellular Localisation | MelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvIsoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinPhotoreceptor Inner SegmentCell ProjectionCiliumPhotoreceptor Outer SegmentIsoform 2: Cell MembraneEndosomeEndoplasmic Reticulum MembraneBasolateral Cell MembraneIsoform 3: Cell MembraneIsoform 4: Cell Membrane |
| Alternative Antibody Pair Names | Basigin antibody pair5f7 antibody pairCollagenase Stimulatory Factor antibody pairExtracellular Matrix Metalloproteinase Inducer antibody pairEmmprin antibody pairHepatoma-Associated Antigen antibody pairHab18g antibody pairLeukocyte Activation Antigen M6 antibody pairOk Blood Group Antigen antibody pairTumor Cell-Derived Collagenase Stimulatory Factor antibody pairTcsf antibody pairCd Antigen Cd147 antibody pairBSG antibody pairUNQ6505 antibody pairPRO21383 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CD147 (CD147) Antibody Pair (STJAP00764)
This antibody pair is designed to detect human CD147 (UniProt P35613) and has confirmed reactivity against human samples only.
This antibody pair has been validated for use in ELISA. It is intended for research use only and has not been validated for diagnostic or therapeutic applications.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Clone IDs are R54-9C-6 and R89-2G-1 for the two components of the pair.
The optimal working dilution for each antibody is to be determined by the end user and should be established through titration in the specific assay format and sample type being used.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided to maintain activity.
Both antibodies in the pair were raised in rabbit against recombinant human CD147 protein. No further detail on the immunogen region is provided in the current product specification.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.