Human CD113 (Nectin-3) protein (Recombinant) (STJP000694)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding extracellular domain of human CD113 (nectin-3) including a C-terminal 6His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human CD113 (Nectin-3)-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | 0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity of Recombinant human CD113 was measured in a functional ELISA assay, immobilized recombinant human CD113 at 1 Mu g/ml ( 100 Mu l/well ) can bind biotinylated Nectin-1 with a linear range of 6-800 Mu g/ml NA |
Target Information
| Gene Symbol | NECTIN3 |
| Gene ID | 25945 |
| Uniprot ID | NECT3_HUMAN |
| Immunogen | Optimized DNA sequence encoding extracellular domain of human CD113 (nectin-3) including a C-terminal 6His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ECD |
Additional Info
| Tissue Specificity | Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. |
| Function | Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types. Plays a role in the morphology of the ciliary body. |
| Protein Name | Nectin-3Cdw113Nectin Cell Adhesion Molecule 3Poliovirus Receptor-Related Protein 3Cd Antigen Cd113 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-420597 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinPostsynaptic Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionAdherens JunctionIn The Auditory EpitheliumSpecificaly Localizes To The Apical Side Of The Lateral Membranes Of Supporting Cells |
| Alternative Protein Names | Nectin-3 proteinCdw113 proteinNectin Cell Adhesion Molecule 3 proteinPoliovirus Receptor-Related Protein 3 proteinCd Antigen Cd113 proteinNECTIN3 proteinPRR3 proteinPVRL3 protein |
Information sourced from Uniprot.org