| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human CD113 (Nectin-3)-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
0.2 Mu m filtered PBS solution, pH7.2. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity of Recombinant human CD113 was measured in a functional ELISA assay, immobilized recombinant human CD113 at 1 Mu g/ml ( 100 Mu l/well ) can bind biotinylated Nectin-1 with a linear range of 6-800 Mu g/ml NA |
| Gene Symbol: |
NECTIN3 |
| Gene ID: |
25945 |
| Uniprot ID: |
NECT3_HUMAN |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of human CD113 (nectin-3) including a C-terminal 6His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. |
| Function | Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. |
| Protein Name | Nectin-3Cdw113Nectin Cell Adhesion Molecule 3Poliovirus Receptor-Related Protein 3Cd Antigen Cd113 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-420597 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinPostsynaptic Cell Membrane |
| Alternative Protein Names | Nectin-3 proteinCdw113 proteinNectin Cell Adhesion Molecule 3 proteinPoliovirus Receptor-Related Protein 3 proteinCd Antigen Cd113 proteinNECTIN3 proteinPRR3 proteinPVRL3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
