Human CCL2/MCP-1 protein (Recombinant) (C-His) (STJP002864)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CCL2/MCP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln24-Thr99) of human CCL2/MCP-1 (Accession #NP_002973.1) fused with and a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CCL2/MCP-1-C-His protein was developed in hek293 cells using the region Gln24-Thr99. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is typically 3.33-13.32 ng/mL. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | CCL2 |
| Gene ID | 6347 |
| Uniprot ID | CCL2_HUMAN |
| Immunogen | Recombinant Human CCL2/MCP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln24-Thr99) of human CCL2/MCP-1 (Accession #NP_002973.1) fused with and a 6×His tag at the C-terminus. |
| Immunogen Region | Gln24-Thr99 |
| Immunogen Sequence | QPDAINAPVTCCYNFTNRKI SVQRLASYRRITSSKCPKEA VIFKTIVAKEICADPKQKWV QDSMDHLDKQTQTPKT |
Additional Info
| Post Translational Modifications | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. N-Glycosylated. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Protein Name | C-C Motif Chemokine 2Hc11Monocyte Chemoattractant Protein 1Monocyte Chemotactic And Activating FactorMcafMonocyte Chemotactic Protein 1Mcp-1Monocyte Secretory Protein JeSmall-Inducible Cytokine A2 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9818026 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | C-C Motif Chemokine 2 proteinHc11 proteinMonocyte Chemoattractant Protein 1 proteinMonocyte Chemotactic And Activating Factor proteinMcaf proteinMonocyte Chemotactic Protein 1 proteinMcp-1 proteinMonocyte Secretory Protein Je proteinSmall-Inducible Cytokine A2 proteinCCL2 proteinMCP1 proteinSCYA2 protein |
Information sourced from Uniprot.org