Human CCL14/HCC-1/HCC-3 protein (Recombinant) (No-Tag) (STJP002965)

SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Recombinant Human CCL14/HCC-1/HCC-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr20-Asn93) of Human CCL14/HCC-1/HCC-3 (Accession #NP_116739.1) fused with His tag at the C-terminu
STJP002965
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General Information

Short DescriptionRecombinant-Human CCL14/HCC-1/HCC-3-No-Tag protein was developed in e.coli using the region Thr20-Asn93. For use in research applications.
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ConjugationUnconjugated
FormulationLyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% Trehalose is added as protectant before lyophilisation.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method<0.01 EU/ug of the protein by LAL method

Target Information

Gene SymbolCCL14
Gene ID6358
Uniprot IDCCL14_HUMAN
ImmunogenRecombinant Recombinant Human CCL14/HCC-1/HCC-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr20-Asn93) of Human CCL14/HCC-1/HCC-3 (Accession #NP_116739.1) fused with His tag at the C-terminu
Immunogen RegionThr20-Asn93
Immunogen SequenceAPLGEVGNYFGVQDAVPFGN VPVLPVDSPVLLSDHLGQSE AGGLPRGPAVTDLDHLKGIL RRRQLYCRTGFHLEIFPNGT IQGTRKDHSRFGILEFISIA VGLVSIRGVDSGLYLGMNEK GELYGSEKLTQECVFREQFE ENWYNTYSSNLYKHVDTGRR YYVALNKDGTPREGTRTKRH QKFTHFLPRPVDPDKVPELY KDILSQS

Additional Info

Tissue Specificity Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
Post Translational Modifications The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated.the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
Function Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Protein Name C-C Motif Chemokine 14
Chemokine Cc-1/Cc-3
Hcc-1/Hcc-3
Hcc-1(1-74
Ncc-2
Small-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74
Database Links
Cellular Localisation Secreted
Alternative Protein Names C-C Motif Chemokine 14 protein
Chemokine Cc-1/Cc-3 protein
Hcc-1/Hcc-3 protein
Hcc-1(1-74 protein
Ncc-2 protein
Small-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74 protein
CCL14 protein
NCC2 protein
SCYA14 protein

Information sourced from Uniprot.org

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